Upload 细胞给药助手-GUI (1).py

细胞给药助手-GUI (1).py

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+import tkinter as tk
+from tkinter import filedialog, messagebox, ttk
+import csv
+
+def calculate():
+    try:
+        total_vol = float(total_volume_entry.get())
+        mw = float(mw_entry.get())
+        ug_ml = float(ugml_entry.get())
+        mm_input = float(mm_entry.get())
+
+        # 单位换算
+        if mw > 0:
+            mmol_per_l = (ug_ml / 1000) / mw * 1000
+            mmol_label.config(text=f"≈ {mmol_per_l:.3f} mM")
+            ugml_back = mm_input * mw
+            ugml_label.config(text=f"≈ {ugml_back:.2f} µg/mL")
+
+        # 解析 stock_solutions
+        stock_lines = stock_text.get("1.0", "end").strip().split("\n")
+        stock_solutions = {}
+        for line in stock_lines:
+            try:
+                c, v = line.strip().split(":")
+                stock_solutions[float(c.strip())] = float(v.strip())
+            except:
+                messagebox.showwarning("格式错误", f"格式错误：{line}")
+                return
+
+        # 目标浓度
+        concs = [float(x.strip()) for x in conc_entry.get().split(",") if x.strip()]
+        results = []
+
+        for fc in concs:
+            best_stock = None
+            min_vol = float('inf')
+            selected_volume = 0
+
+            if fc == 0:
+                results.append([fc, "无", 0, total_vol, "对照组"])
+                continue
+
+            for sc, max_vol in stock_solutions.items():
+                if sc >= fc:
+                    v1 = (fc * total_vol) / sc
+                    if v1 <= max_vol and v1 < min_vol:
+                        min_vol = v1
+                        best_stock = sc
+                        selected_volume = round(v1, 2)
+
+            if best_stock is None:
+                results.append([fc, "无法满足", "-", "-", "浓度过高或储备液不足"])
+            else:
+                media_vol = round(total_vol - selected_volume, 2)
+                results.append([fc, f"{best_stock} mM", selected_volume, media_vol, "✓"])
+
+        # 展示结果
+        for row in result_tree.get_children():
+            result_tree.delete(row)
+        for row in results:
+            result_tree.insert("", "end", values=row)
+
+    except Exception as e:
+        messagebox.showerror("错误", str(e))
+
+def export_csv():
+    path = filedialog.asksaveasfilename(defaultextension=".csv", filetypes=[("CSV 文件", "*.csv")])
+    if not path:
+        return
+    with open(path, "w", newline='') as f:
+        writer = csv.writer(f)
+        writer.writerow(["终浓度 (mM)", "使用储备液", "加药体积 (µL)", "培养基体积 (µL)", "状态"])
+        for row_id in result_tree.get_children():
+            writer.writerow(result_tree.item(row_id)['values'])
+
+root = tk.Tk()
+root.title("🧪 自动配药计算工具 GUI 版")
+
+tk.Label(root, text="每孔总体积 (µL)").grid(row=0, column=0)
+total_volume_entry = tk.Entry(root)
+total_volume_entry.insert(0, "2000")
+total_volume_entry.grid(row=0, column=1)
+
+tk.Label(root, text="分子量 (g/mol)").grid(row=1, column=0)
+mw_entry = tk.Entry(root)
+mw_entry.insert(0, "194.19")
+mw_entry.grid(row=1, column=1)
+
+tk.Label(root, text="µg/mL 浓度").grid(row=2, column=0)
+ugml_entry = tk.Entry(root)
+ugml_entry.insert(0, "0.0")
+ugml_entry.grid(row=2, column=1)
+
+mmol_label = tk.Label(root, text="≈ 0.000 mM")
+mmol_label.grid(row=2, column=2)
+
+tk.Label(root, text="mM 浓度").grid(row=3, column=0)
+mm_entry = tk.Entry(root)
+mm_entry.insert(0, "0.0")
+mm_entry.grid(row=3, column=1)
+
+ugml_label = tk.Label(root, text="≈ 0.00 µg/mL")
+ugml_label.grid(row=3, column=2)
+
+tk.Label(root, text="储备液设置 (mM:µL)").grid(row=4, column=0)
+stock_text = tk.Text(root, height=4, width=30)
+stock_text.insert("1.0", "2: 1000\n5: 1000\n20: 1000")
+stock_text.grid(row=4, column=1, columnspan=2)
+
+tk.Label(root, text="目标浓度列表 (mM, 逗号分隔)").grid(row=5, column=0)
+conc_entry = tk.Entry(root, width=30)
+conc_entry.insert(0, "0,0.5,1,2,5,10")
+conc_entry.grid(row=5, column=1, columnspan=2)
+
+tk.Button(root, text="计算", command=calculate).grid(row=6, column=1)
+tk.Button(root, text="导出 CSV", command=export_csv).grid(row=6, column=2)
+
+cols = ["终浓度 (mM)", "使用储备液", "加药体积 (µL)", "培养基体积 (µL)", "状态"]
+result_tree = ttk.Treeview(root, columns=cols, show="headings", height=8)
+for col in cols:
+    result_tree.heading(col, text=col)
+    result_tree.column(col, width=120)
+result_tree.grid(row=7, column=0, columnspan=3, pady=10)
+
+root.mainloop()